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Molecular interactions of graphene oxide with human blood plasma proteins

TitleMolecular interactions of graphene oxide with human blood plasma proteins
Publication TypeJournal Article
Year of Publication2016
Authors, Loh Kian Ping, and Lim Chwee Teck
JournalNanoscale
Volume8
Pagination9425–9441
Date Published2016
Abstract

We investigate the molecular interactions between graphene oxide (GO) and human blood plasma proteins. To gain an insight into the bio-physico-chemical activity of GO in biological and biomedical applications, we performed a series of biophysical assays to quantify the molecular interactions between GO with different lateral size distributions and the three essential human blood plasma proteins. We elucidate the various aspects of the GO-protein interactions, particularly, the adsorption, binding kinetics and equilibrium, and conformational stability, through determination of quantitative parameters, such as GO-protein association constants, binding cooperativity, and the binding-driven protein structural changes. We demonstrate that the molecular interactions between GO and plasma proteins are significantly dependent on the lateral size distribution and mean lateral sizes of the GO nanosheets and their subtle variations may markedly influence the GO-protein interactions. Consequently, we propose the existence of size-dependent molecular interactions between

DOI10.1039/c6nr01697a

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